Dionex 3000 Manual

The Dionex 3000 is a high-performance ion chromatography system designed for analytical laboratories, featuring advanced separation capabilities, automated sample handling, precise flow control, conductivity detection, and comprehensive data analysis software. Below are key sections for safety, features, installation, setup, operation, and troubleshooting.

Features at a Glance

Key components: High-pressure pump, conductivity detector, autosampler, chromatography column, eluent generator, and data system interface.

Feature	Description
High-pressure pump	Precise flow control up to 5000 psi
Conductivity detector	High-sensitivity detection with temperature control
Autosampler	Automated sample injection for up to 120 vials
Eluent generator	On-demand eluent production for consistent results
Column oven	Temperature control from 5°C to 80°C
Data system interface	USB and Ethernet connectivity
Power switch	Main system power control
Emergency stop	Immediate system shutdown
Flow control	Adjustable flow rates from 0.1 to 5.0 mL/min
Pressure monitoring	Real-time pressure display and alarms

Installation

Unpack and inspect contents. Place on stable laboratory bench.

1. Connect eluent lines: Attach eluent reservoir to pump inlet using specified tubing.
2. Install chromatography column: Connect column between pump outlet and detector inlet.
3. Connect waste line: Attach tubing from detector outlet to waste container.
4. Power connection: Plug into appropriate laboratory power outlet.
5. Computer connection: Connect via USB or Ethernet to data system.

WARNING! System operates under high pressure; ensure all connections are secure before operation. Use only specified consumables.

First-Time Setup

Power on system; initialize software and configure parameters.

1. System priming: Purge pump and lines to remove air bubbles.
2. Column conditioning: Run system with mobile phase for 30 minutes.
3. Detector calibration: Perform baseline calibration according to protocol.
4. Method development: Set flow rate, temperature, and detection parameters.
5. Sample sequence: Program autosampler sequence for batch analysis.

CAUTION! Always wear appropriate personal protective equipment when handling chemicals.

Specifications

• Flow Rate Range: 0.1 to 5.0 mL/min
• Pressure Limit: 5000 psi maximum operating pressure
• Temperature Control: 5°C to 80°C (±0.1°C)
• Detection: Conductivity with temperature compensation
• Sample Capacity: 120 vials (2 mL standard)
• Data Output: USB, Ethernet, analog output
• Power Requirements: 100-240V AC, 50/60Hz
• Dimensions: 55 cm W × 45 cm D × 45 cm H
• Weight: 35 kg

Controls and Settings

Access system controls via front panel interface or software application.

Front Panel: POWER, EMERGENCY STOP, FLOW CONTROL, TEMPERATURE SET, PRESSURE DISPLAY.

Software Interface: Method parameters (flow rate, temperature, run time), detection settings (range, output), data acquisition (sampling rate, filter), sequence programming (injection volume, vial positions). System status: Pressure monitoring, temperature readout, error logging.

Connecting Devices

Eluent reservoir: Connect to pump inlet. Column: Connect between pump and detector. Waste: Connect from detector outlet. Computer: USB/Ethernet connection.

1. Turn off system before making connections.
2. Use only specified tubing and fittings.
3. Ensure all connections are finger-tight plus 1/4 turn.
4. Pressure test system before sample analysis.

Tip: Label all connections for easy identification during maintenance.

Operating Procedures

Standard operating protocol for analytical runs.

1. System preparation: Prime pump, equilibrate column, establish stable baseline.
2. Sample loading: Place vials in autosampler, ensure proper alignment.
3. Method selection: Choose appropriate method for analyte separation.
4. Run initiation: Start sequence from software interface.
5. Monitoring: Observe pressure, baseline, and peak separation during run.
6. Data analysis: Review chromatograms, integrate peaks, generate reports.
7. System shutdown: Flush with appropriate storage solution.

WARNING! Never exceed maximum pressure rating. Monitor system throughout operation.

Maintenance Features

Preventive maintenance schedule: Daily system checks, weekly seal replacement, monthly detector service, quarterly pump maintenance. System diagnostics: Built-in self-test routines, performance verification protocols.

Care & Cleaning

Power off before cleaning. Use isopropanol for exterior surfaces. Flush system with appropriate solvents for internal cleaning.

CAUTION! Use only compatible solvents. Follow waste disposal regulations.

Regulatory Information

CE marked: Complies with European Union safety standards. FDA 21 CFR Part 11: Software meets electronic records requirements. ISO 9001: Quality management system certified. For technical support, contact Dionex customer service at 1-800-723-1161 or visit www.thermofisher.com/dionex.

Troubleshooting

Symptom	Possible Cause	Corrective Action
High pressure	Column blockage	Check for particulates; replace inlet frit; flush column.
No flow	Pump seal failure	Replace pump seals; prime system; check for air bubbles.
Baseline noise	Contaminated eluent	Prepare fresh eluent; clean detector cell; check grounding.
Peak tailing	Column degradation	Condition column; check mobile phase pH; replace if necessary.
Autosampler error	Misaligned needle	Check needle position; clean needle; recalibrate position.

Reset: Software > System > Reset to factory defaults if available.

Service: 1-800-723-1161; www.thermofisher.com/dionex