Dionex AS AP Service Manual

The Dionex AS AP is an advanced automated sample preparation system designed for chromatographic analysis applications. This system features automated sample handling, precision fluid delivery, integrated temperature control, multiple solvent channels, programmable methods, and compatibility with various Dionex chromatography systems. Below are key sections for safety, features, installation, setup, operation, maintenance, and troubleshooting.

Features at a Glance

Key components: Automated sample tray, Solvent delivery system, Temperature-controlled compartment, User interface panel, Communication ports, Waste collection system.

Installation

Unpack and inspect contents. Place on stable, level laboratory bench.

1. Position the system: Ensure adequate clearance for ventilation and access.
2. Connect solvent lines: Install solvent reservoirs and connect tubing to appropriate ports.
3. Connect waste system: Attach waste container and ensure proper drainage.
4. Power connection: Plug into appropriate laboratory power outlet.
5. Communication setup: Connect to computer or network if required.

WARNING! System contains sensitive electronic components; handle with care. Ensure proper grounding.

First-Time Setup

Power on system; follow initialization procedure.

1. System initialization: Allow system to complete self-test and calibration.
2. Solvent priming: Prime all solvent lines to remove air bubbles.
3. Method configuration: Set up initial analysis methods or load predefined methods.
4. Communication setup: Configure network settings and computer interface.
5. User accounts: Set up operator accounts with appropriate access levels.

CAUTION! Use only recommended solvents and reagents. Follow laboratory safety protocols.

Specifications

• Sample Capacity: 96-position automated tray
• Temperature Range: 4°C to 40°C (±0.5°C)
• Solvent Channels: 4 independent channels
• Flow Rate Range: 0.1 mL/min to 10 mL/min
• Pressure Range: 0 to 6000 psi
• Injection Volume: 1 μL to 100 μL
• Communication: USB 2.0, Ethernet 10/100, RS-232
• Power Requirements: 100-240V AC, 50/60Hz, 500W max
• Dimensions: 600mm W × 500mm D × 400mm H
• Weight: 35 kg

Controls and Settings

Access system controls via touchscreen interface or remote software.

Front Panel: Power button, Emergency stop, Touchscreen display, Status indicators.

Software Interface: Method setup, Sample queue management, Real-time monitoring, Data logging, System diagnostics. Temperature Control: Setpoint adjustment, Ramp programming, Stability monitoring. Solvent Management: Channel selection, Flow rate programming, Gradient methods, Purge functions. Sample Handling: Tray position control, Injection volume, Wash cycles, Needle positioning.

Connecting Devices

Connect to chromatography systems, computers, and peripheral devices.

1. Turn off system before making connections.
2. Connect to chromatography system: Use appropriate interface cables.
3. Computer connection: Connect via USB or Ethernet for remote control.
4. Network setup: Configure IP settings for laboratory network integration.
5. Peripheral devices: Connect barcode readers or additional accessories.

Tip: Use shielded cables for communication connections to minimize interference.

Operating Procedures

Standard operating sequence for sample analysis.

1. System preparation: Prime solvents, equilibrate temperature, initialize system.
2. Sample loading: Place samples in tray, verify barcode reading if applicable.
3. Method selection: Choose appropriate analysis method from stored methods.
4. Run initiation: Start analysis sequence, monitor progress.
5. Data collection: System automatically collects and stores analysis data.
6. System shutdown: Complete wash cycles, return to standby temperature.
7. Sample retrieval: Remove processed samples from tray.

WARNING! Follow proper laboratory procedures for handling chemical samples.

Maintenance Procedures

Routine maintenance schedule and procedures.

1. Daily: Check solvent levels, inspect for leaks, clean exterior surfaces.
2. Weekly: Perform system performance verification, clean sample needle.
3. Monthly: Replace solvent filters, calibrate temperature sensors.
4. Quarterly: Perform comprehensive system calibration, replace seals as needed.
5. Annual: Professional service and certification recommended.

CAUTION! Always power off system before performing maintenance.

Care & Cleaning

Proper cleaning procedures for system components.

Exterior surfaces: Use mild detergent and soft cloth. Sample tray: Clean with appropriate laboratory cleaners. Solvent lines: Flush with compatible solvents. Optical components: Use lens cleaning materials only.

CAUTION! Do not use abrasive cleaners or solvents that may damage components.

Regulatory Information

CE Mark: Complies with European Union safety standards. FDA: Meets requirements for laboratory instrumentation. RoHS: Compliant with Restriction of Hazardous Substances directive. Copyright © 2024 Thermo Fisher Scientific; trademarks of Thermo Fisher Scientific Inc. For technical support, visit www.thermofisher.com or contact 1-800-532-4752.

Troubleshooting

Reset: System menu > Maintenance > System reset if available.

Service: 1-800-532-4752; www.thermofisher.com