Dionex AS Autosampler Manual

The Dionex AS Autosampler is a precision analytical instrument designed for automated sample injection in chromatography systems. It features high-precision injection capabilities, temperature-controlled sample compartments, programmable injection sequences, compatibility with various vial types, and seamless integration with Dionex chromatography systems. Below are key sections for safety, features, installation, setup, operation, and troubleshooting.

1. Features at a Glance 2. Installation 3. First-Time Setup 4. Specifications 5. Controls and Settings 6. Connecting Devices 7. Sample Processing 8. Maintenance Features 9. Care & Cleaning 10. Regulatory Information 11. Troubleshooting

Features at a Glance

Key components: Precision injection system, Temperature-controlled sample compartment, Control panel, Vial tray, Syringe assembly, Communication ports.

Feature	Description
Injection System	High-precision syringe for accurate sample delivery
Sample Compartment	Temperature-controlled from 4°C to 40°C
Vial Capacity	Supports up to 120 standard vials
Control Panel	LCD display with intuitive navigation buttons
Communication Ports	RS-232 and USB for system integration
Power Input	100-240V AC, 50/60Hz universal power supply
Wash Station	Automated needle wash between injections
Method Storage	Stores up to 10 user-defined methods
Injection Volume	Programmable from 0.1μL to 100μL
Carryover	<0.1% typical performance

Installation

Unpack and inspect contents. Place on stable, level surface near chromatography system.

Position unit: Ensure adequate clearance for vial tray movement and door opening.
Connect to power: Use provided power cord to connect to appropriate outlet.
Connect to chromatography system: Use RS-232 or USB cable as required.
Install vial tray: Load appropriate tray for your application.
Prime system: Follow initial priming procedure in setup menu.

WARNING! Instrument contains sensitive electronic components; handle with care. Ensure proper grounding.

First-Time Setup

Power on autosampler; follow on-screen initialization procedure.

System initialization: Allow system to complete self-test and calibration.
Set communication parameters: Configure RS-232 or USB settings for system integration.
Load method: Create or load injection method with desired parameters.
Set temperature: Configure sample compartment temperature as required.
Prime fluidics: Run priming sequence to remove air bubbles from system.

CAUTION! Use only recommended solvents and vials. Avoid exposure to corrosive chemicals.

Specifications

Injection Volume Range: 0.1μL to 100μL
Precision: <1% RSD typical
Temperature Control: 4°C to 40°C (±0.5°C)
Vial Capacity: Up to 120 positions
Injection Types: Full loop, partial loop, micro-volume
Carryover: <0.1% typical
Communication: RS-232, USB 2.0
Power Requirements: 100-240V AC, 50/60Hz
Dimensions: Standard benchtop footprint
Weight: Approximately 15 kg

Controls and Settings

Access functions via front panel controls or software interface. Front Panel: POWER, MENU, ENTER, NAVIGATION arrows, START/STOP.

Software Interface: Method editor, Sequence programming, System status, Calibration, Diagnostics.

Method Parameters: Injection volume, Wash cycles, Temperature, Injection speed, Sample draw speed. System Settings: Communication parameters, Date/time, User access levels, System diagnostics. Maintenance: Prime, Purge, Needle alignment, System calibration.

Connecting Devices

Chromatography system: RS-232 or USB connection. Computer: USB for software control. External devices: Contact closure inputs/outputs.

Turn off all devices before making connections.
Use shielded cables for communication connections.
Verify communication settings match between devices.
Test communication before starting analysis.

Tip: Use manufacturer-recommended cables and connectors for optimal performance.

Sample Processing

Supports various injection modes and sequence programming.

Load samples: Place vials in designated positions in tray.
Create sequence: Program injection order, volumes, and parameters.
Start analysis: Initiate sequence from front panel or software.
Monitor progress: Watch status indicators and progress display.
Pause/resume: Stop sequence temporarily if needed.
Method editing: Modify parameters during sequence if required.
Data output: Injection events logged for data system integration.
Shutdown: Properly shut down system after completion.

WARNING! Do not open door during operation. Ensure proper solvent levels.

Maintenance Features

Automated maintenance: Needle wash between injections, System self-diagnostics, Leak detection. User maintenance: Regular seal replacement, Syringe cleaning, Tray alignment verification.

Care & Cleaning

Power off before cleaning. Use mild detergent and water for exterior surfaces. Follow manufacturer instructions for fluid path cleaning.

CAUTION! Use only compatible solvents for fluid path cleaning. Avoid abrasive cleaners on surfaces.

Regulatory Information

CE Mark: Complies with European Union directives. FCC Part 15: Class A digital device. UL/CSA: Certified for electrical safety. For technical support, contact Dionex Technical Support or visit www.thermofisher.com/dionex.

Troubleshooting

Symptom	Possible Cause	Corrective Action
No communication	Cable/configuration	Check cable connections; verify communication settings; restart system.
Injection volume inaccurate	Air bubbles/calibration	Prime system to remove bubbles; perform syringe calibration.
Needle alignment error	Mechanical obstruction	Check for obstructions; perform needle alignment procedure.
Temperature fluctuation	Ambient conditions	Ensure adequate clearance for ventilation; check room temperature stability.
Leak detection error	Seal wear/connection	Inspect seals and connections; replace worn components.

Reset: System menu > Maintenance > System reset if required.

Service: Contact authorized Dionex service provider for technical support.