Dionex AS-DV Autosampler Manual

The Dionex AS-DV Autosampler is an advanced automated sample handling system designed for chromatographic analysis applications. It features precise sample injection, temperature-controlled sample storage, programmable injection sequences, compatibility with various vial types, and integration with Dionex chromatography systems. Below are key sections for safety, features, installation, setup, operation, and troubleshooting.

1. Features at a Glance 2. Installation 3. First-Time Setup 4. Specifications 5. Controls and Settings 6. Connecting Devices 7. Sample Preparation and Loading 8. Programming Methods 9. Maintenance & Cleaning 10. Regulatory Information 11. Troubleshooting

Features at a Glance

Key components: Sample tray with temperature control, Injection port, Syringe assembly, Control panel, Communication ports, Power connector.

FeatureDescription
Sample TrayTemperature-controlled compartment for sample vials
Injection PortPrecision injection mechanism for accurate sample delivery
Syringe AssemblyProgrammable volume injection system
Control PanelLCD display with navigation buttons
Communication PortsRS-232 and Ethernet for system integration
Power ConnectorFor main power connection
Temperature Control4°C to 40°C sample compartment
Vial CapacityUp to 120 standard vials
Injection Volume0.1 to 100 μL programmable range
Wash StationAutomated needle washing between injections

Installation

Unpack and inspect contents. Place on stable, level surface near chromatography system.

  1. Position unit: Ensure adequate clearance for tray movement and door opening.
  2. Connect to power: Use provided power cord to AC outlet.
  3. Connect to chromatography system: Use appropriate interface cables.
  4. Install sample tray: Load empty vial rack into compartment.
  5. Prime fluidics: Follow initial startup procedure.

WARNING! Ensure proper grounding. Avoid locations with excessive vibration or temperature fluctuations.

First-Time Setup

Power on autosampler; follow initialization sequence.

  1. System initialization: Allow self-test to complete.
  2. Set communication parameters: Configure RS-232 or Ethernet settings.
  3. Calibrate injection volume: Perform syringe calibration procedure.
  4. Set temperature: Configure sample compartment temperature.
  5. Test operation: Run diagnostic sequence to verify performance.

CAUTION! Use only compatible solvents and vials. Follow chemical compatibility guidelines.

Specifications

Controls and Settings

Access functions via front panel or software interface. Front Panel: POWER, MENU, ENTER, NAVIGATION arrows, START/STOP.

Software Control: Method programming, sequence setup, temperature control, injection parameters, system status monitoring.

Basic Settings: Injection volume, wash cycles, needle depth, injection speed, tray temperature. Advanced Settings: Method sequences, calibration factors, communication parameters, system diagnostics.

Connecting Devices

Connect to chromatography system, computer, and power source.

  1. Turn off all devices before connecting.
  2. Connect to HPLC/IC system: Use appropriate interface cable.
  3. Connect to computer: Use RS-232 or Ethernet for remote control.
  4. Connect solvent lines: Ensure proper tubing connections.
  5. Verify connections: Power on and test communication.

Tip: Use shielded cables for communication connections to prevent interference.

Sample Preparation and Loading

Prepare samples according to analytical method requirements.

  1. Prepare vials: Use compatible vials with proper septa.
  2. Load samples: Place vials in tray according to sequence.
  3. Seal vials: Ensure proper crimping or capping.
  4. Program sequence: Enter vial positions and injection parameters.
  5. Start analysis: Initiate automated sequence.
  6. Monitor progress: Watch for errors or system alerts.

WARNING! Handle biological and chemical samples with appropriate safety precautions.

Programming Methods

Create and save method sequences for automated operation.

  1. Access method editor: From main menu or software.
  2. Define injection parameters: Volume, speed, wash cycles.
  3. Set sequence: Specify vial order and replicates.
  4. Configure temperature: Set compartment temperature.
  5. Save method: Store for future use.
  6. Validate method: Test with standards before samples.

Note: Methods can include calibration standards, quality controls, and unknown samples.

Maintenance & Cleaning

Regular maintenance ensures optimal performance and longevity.

  1. Daily: Check for leaks, clean exterior surfaces.
  2. Weekly: Perform needle wash, check syringe function.
  3. Monthly: Replace seals and septa as needed.
  4. As needed: Calibrate injection volume, clean sample tray.

CAUTION! Use only recommended cleaning solvents. Power off before maintenance.

Regulatory Information

CE Mark: Complies with European Union safety standards. FDA: Suitable for use in regulated laboratories. Copyright © 2025 Thermo Fisher Scientific. For technical support, visit www.thermofisher.com or contact 1-800-532-4752.

Troubleshooting

SymptomPossible CauseCorrective Action
No injectionSyringe blockage/air bubblesPrime syringe, check for obstructions, verify solvent flow
Poor precisionWorn seals/contaminationReplace syringe seals, clean injection port
Communication errorCable/configuration issuesCheck cables, verify communication settings
Temperature fluctuationEnvironmental factorsEnsure stable room temperature, check compressor
Sample carryoverInadequate washingIncrease wash volume/cycles, check wash solvent

Reset: Power cycle unit or use system reset function.

Service: 1-800-532-4752; www.thermofisher.com

Dionex AS-DV Autosampler Manual © - All Rights Reserved 2025