Dionex ASE 200 Manual

The Dionex ASE 200 is an accelerated solvent extraction system designed for rapid extraction of solid and semi-solid samples. It uses high temperature and pressure to significantly reduce extraction time and solvent consumption compared to traditional methods. Below are key sections for safety, features, installation, setup, operation, and troubleshooting.

Features at a Glance

Key components: Extraction cell, solvent delivery system, heating oven, collection vials, pressure control system, and user interface.

Feature	Description
Extraction Cell	Stainless steel cell for sample containment
Solvent Delivery	Automated solvent delivery system
Heating Oven	Temperature range 40-200°C
Pressure Control	Operating pressure up to 2000 psi
Collection Vials	40mL vials for extract collection
User Interface	LCD display with keypad control
Safety Features	Pressure relief valves, temperature monitoring
Solvent Options	Compatible with various organic solvents
Automation	Programmable extraction methods
Data Storage	Method and run data storage

Installation

Unpack and inspect contents. Place on stable laboratory bench.

1. Location: Install in well-ventilated area with fume hood.
2. Power: Connect to appropriate power supply (120V/240V).
3. Solvent Lines: Connect solvent bottles with appropriate tubing.
4. Gas Supply: Connect nitrogen gas for pressurization.
5. Waste: Connect waste collection system.

WARNING! System operates under high pressure and temperature; follow all safety protocols during installation.

First-Time Setup

Power on system; follow initialization procedure.

1. System Check: Run diagnostic tests to verify all components.
2. Solvent Prime: Prime solvent lines to remove air bubbles.
3. Pressure Test: Perform leak test at operating pressure.
4. Method Setup: Program standard extraction methods.
5. Calibration: Calibrate temperature and pressure sensors.

CAUTION! Wear appropriate PPE when handling solvents and samples.

Specifications

• Temperature Range: 40-200°C
• Pressure Range: Up to 2000 psi
• Extraction Time: 12-20 minutes per sample
• Solvent Consumption: 15-40 mL per extraction
• Sample Size: 1-100 grams
• Cell Volume: 1, 5, 11, 22, 33 mL options
• Power Requirements: 120V/240V, 50/60Hz
• Dimensions: Standard benchtop unit
• Weight: Approximately 45 kg

Controls and Settings

Access control panel via front interface. Main Controls: POWER, START/STOP, MENU, ENTER, NAVIGATION arrows.

Menu Options: Method Setup, Run Control, System Settings, Diagnostics.

Extraction Parameters: Temperature, Pressure, Heat Time, Static Time, Flush Volume, Purge Time. System: Language, Units, Date/Time, Data Management. Safety: Pressure Limits, Temperature Limits, Emergency Stop.

Connecting Devices

Solvent Bottles: Connect via solvent lines. Gas Supply: Nitrogen gas connection. Waste: Waste collection system.

1. Turn off system before making connections.
2. Use appropriate tubing and fittings for solvents.
3. Ensure all connections are tight and leak-free.
4. Verify gas pressure meets requirements.

Tip: Label all solvent lines clearly to avoid cross-contamination.

Operating Procedures

Standard extraction procedure for solid samples.

1. Sample Preparation: Weigh and load sample into extraction cell.
2. Cell Assembly: Assemble cell with filters and end caps.
3. Load Cell: Place cell in carousel or extraction position.
4. Select Method: Choose appropriate extraction method.
5. Start Extraction: Press START to begin automated process.
6. Monitor: Watch pressure and temperature during extraction.
7. Collection: Extract collects in designated vials.
8. Shutdown: Properly shut down system after completion.

WARNING! Do not open system during operation; wait for pressure and temperature to return to safe levels.

Safety Features

Pressure Relief: Automatic pressure relief valves. Temperature Monitoring: Over-temperature protection. Emergency Stop: Immediate system shutdown. Leak Detection: System monitors for solvent leaks.

Maintenance & Cleaning

Regular maintenance ensures optimal performance and longevity.

1. Daily: Clean exterior surfaces, check solvent levels.
2. Weekly: Clean collection vials, check tubing for wear.
3. Monthly: Replace filters, perform system calibration.
4. Quarterly: Comprehensive system inspection and maintenance.

CAUTION! Always depressurize system before performing maintenance.

Regulatory Information

CE Mark: Complies with European Union safety standards. UL Listed: Meets UL safety requirements. FCC Part 15: Class A digital device. For technical support, contact Dionex Technical Support or visit www.thermofisher.com/dionex.

Troubleshooting

Symptom	Possible Cause	Corrective Action
Pressure not building	Leaks/gas supply	Check all connections; verify gas pressure; inspect seals.
Temperature unstable	Heater/sensor	Check heater operation; calibrate temperature sensor.
Solvent not flowing	Clogged lines/pump	Check for obstructions; prime solvent lines; inspect pump.
Error messages	System faults	Note error code; consult manual; contact support if persistent.
Poor extraction	Method parameters	Optimize temperature, pressure, and time settings.

Reset: System > Maintenance > Reset to factory defaults if needed.

Service: Contact Dionex Technical Support for professional service.