Dionex ASE 350 Service Manual

The Dionex ASE 350 is an accelerated solvent extraction system designed for automated extraction of solid and semi-solid samples. This system utilizes high temperature and pressure to rapidly extract analytes from various matrices, providing faster extraction times and reduced solvent consumption compared to traditional methods. Below are key sections for safety, features, installation, operation, maintenance, and troubleshooting.

1. Features at a Glance 2. Installation 3. First-Time Setup 4. Specifications 5. Controls and Settings 6. Connecting Accessories 7. Operating Procedures 8. Maintenance Schedule 9. Care & Cleaning 10. Regulatory Information 11. Troubleshooting

Features at a Glance

Key components: Solvent controller, extraction cells, collection vials, pressure system, heating oven, and user interface.

Feature	Description
Solvent Controller	Manages up to 4 different solvents with automated mixing
Extraction Cells	Available in 1mL, 5mL, 11mL, 22mL, and 33mL sizes
Collection Vials	40mL and 60mL vials with optional rack systems
Pressure System	Operates up to 1500 psi with nitrogen gas
Heating Oven	Temperature range 40°C to 200°C
User Interface	LCD display with keypad for method programming
Safety Features	Pressure relief valves, leak detection, and temperature monitoring
Method Storage	Up to 10 user-defined methods
Automation	Sequential extraction of up to 24 samples
Data Output	RS-232 interface for data transfer

Installation

Unpack and inspect contents. Place on stable laboratory bench.

Position system: Ensure adequate clearance for ventilation and access.
Connect solvent lines: Attach solvent bottles to solvent controller with appropriate tubing.
Connect nitrogen supply: Attach nitrogen regulator to gas inlet (1500 psi maximum).
Connect waste line: Route waste tubing to appropriate waste container.
Power connection: Plug into 120V AC power outlet with proper grounding.

WARNING! System operates under high pressure and temperature. Ensure all connections are secure before operation.

First-Time Setup

Power on system; follow startup sequence.

System initialization: Allow system to complete self-test and pressure check.
Solvent priming: Prime each solvent line to remove air bubbles.
Pressure test: Perform initial pressure test at 1500 psi for 5 minutes.
Method programming: Create or load extraction methods using keypad interface.
Test extraction: Run test extraction with reference material to verify performance.

CAUTION! Wear appropriate personal protective equipment when handling solvents.

Specifications

Extraction Cells: 1mL, 5mL, 11mL, 22mL, 33mL
Pressure Range: 500 to 1500 psi
Temperature Range: 40°C to 200°C
Heating Time: < 5 minutes to 100°C
Extraction Time: 12-18 minutes per sample
Solvent Consumption: 15-45 mL per extraction
Collection Vials: 40mL and 60mL
Power Requirements: 120V AC, 50/60Hz, 10A
Dimensions: 56cm W × 48cm D × 46cm H
Weight: 34 kg

Controls and Settings

Access system functions via front panel keypad and LCD display.

Main Controls: POWER, START/STOP, METHOD, SETUP, STATUS, PRINT

Method Parameters: Temperature (40-200°C), Pressure (500-1500 psi), Heat Time (1-99 min), Static Time (1-99 min), Flush Volume (30-150%), Purge Time (30-999 sec), Cycles (1-5), Solvent Mixing Ratios.

System Settings: Date/Time, Pressure Units (psi/bar), Temperature Units (°C/°F), Communication Settings, System Diagnostics.

Connecting Accessories

Solvent bottles: Use approved solvent containers with proper venting. Nitrogen supply: High purity nitrogen (99.999%) with two-stage regulator. Waste collection: Chemical-resistant container with adequate capacity.

Turn off system before connecting accessories.
Use only Dionex-approved tubing and fittings.
Verify all connections are leak-free before operation.
Ensure waste container is properly vented and labeled.

Tip: Regularly inspect solvent lines and replace if showing signs of wear or degradation.

Operating Procedures

Standard extraction procedure for solid samples.

Sample preparation: Weigh and load sample into extraction cell with appropriate dispersant.
Cell assembly: Assemble cell with end caps and place in carousel.
Method selection: Choose pre-programmed method or create new method.
Start extraction: Press START and monitor system status.
Collection: Extracts collected in specified vials in collection tray.
System cleanup: Run solvent flush cycle between different sample types.
Shutdown: Perform proper shutdown procedure including pressure release.

WARNING! Always depressurize system before opening cell compartments.

Maintenance Schedule

Regular maintenance ensures optimal performance and extends system life.

Maintenance Task	Frequency	Procedure
Solvent line inspection	Weekly	Check for leaks, cracks, or discoloration
Seal replacement	Every 6 months	Replace all O-rings and seals
Pressure test	Monthly	Verify system holds 1500 psi for 10 minutes
Heater calibration	Annually	Verify temperature accuracy with calibrated thermometer
System cleaning	After each use	Flush with appropriate solvents

Care & Cleaning

Power off and depressurize before cleaning. Use appropriate solvents for flushing. Wipe exterior with mild detergent and soft cloth.

CAUTION! Use only compatible solvents. Never use abrasive cleaners on system surfaces.

Regulatory Information

UL/CSA certified: Complies with laboratory equipment safety standards. CE marked: Meets European Union directives. Manufactured under ISO 9001 quality system. For technical support, contact Dionex Technical Services at 1-800-723-1161 or visit www.thermofisher.com/dionex.

Troubleshooting

Symptom	Possible Cause	Corrective Action
Pressure not building	Gas supply/leaks	Check nitrogen supply; inspect for leaks in fittings and seals
Temperature not reached	Heater failure	Verify heater operation; check temperature sensor
Solvent delivery issues	Clogged lines/pump	Prime solvent lines; check pump operation; replace filters
Leak detection error	Seal failure	Replace O-rings and seals; verify proper cell assembly
System communication error	Cable/interface	Check RS-232 connections; restart system

Reset: Power cycle system; perform system reset in SETUP menu.

Service: 1-800-723-1161; www.thermofisher.com/dionex