Dionex Chromeleon 7 Manual

Dionex Chromeleon 7 is a comprehensive chromatography data system (CDS) software platform for instrument control, data acquisition, processing, and reporting. It supports HPLC, UHPLC, GC, and IC systems with advanced features including automated method development, compliance tools for regulated environments, multi-dimensional chromatography, and extensive data management capabilities. Below are key sections covering installation, features, operation, and troubleshooting.

1. Features at a Glance 2. System Requirements 3. Installation Process 4. Specifications 5. Controls and Interface 6. Instrument Configuration 7. Method Development 8. Data Processing 9. Compliance Features 10. Regulatory Information 11. Troubleshooting

Features at a Glance

Key components: Centralized instrument control, automated method development, compliance tools, data management system, and reporting capabilities.

Feature	Description
Instrument Control	Centralized control for HPLC, UHPLC, GC, and IC systems
Method Development	Automated method scouting and optimization
Compliance Tools	21 CFR Part 11 compliance with audit trails and electronic signatures
Data Management	Secure data storage and retrieval with version control
Reporting	Customizable reports with templates and automated generation
Multi-dimensional Analysis	Support for 2D-LC and comprehensive chromatography
Workflow Automation	Sequence-based automation for routine analyses
Data Processing	Advanced peak integration and quantification algorithms
System Suitability	Automated system suitability testing and reporting
Remote Access	Web-based remote monitoring and control capabilities

System Requirements

Ensure your system meets minimum requirements before installation.

Operating System: Windows 10/11 (64-bit) or Windows Server 2019/2022
Processor: Intel Core i5 or equivalent, 2.5 GHz or higher
Memory: 8 GB RAM minimum (16 GB recommended)
Storage: 50 GB free disk space for installation
Display: 1920x1080 resolution minimum
Network: Gigabit Ethernet for multi-user environments

WARNING! Ensure all system requirements are met before installation to avoid performance issues.

Installation Process

Follow these steps for successful Chromeleon 7 installation.

Pre-installation: Disable antivirus software temporarily; ensure administrator privileges
Run installer: Execute Setup.exe from installation media
Database setup: Configure SQL Server database connection
License activation: Enter license key or connect to license server
Instrument drivers: Install required instrument communication drivers
Verification: Run system verification tests post-installation

CAUTION! Back up existing data before installation. Follow installation wizard prompts carefully.

Specifications

Software Version: Chromeleon 7.3.1
Supported Instruments: Thermo Scientific HPLC, UHPLC, GC, IC systems
Data Formats: Native .cdf, .raw; import/export to .csv, .pdf
Compliance: 21 CFR Part 11 compliant with configurable security
Database: Microsoft SQL Server 2019/2022
Networking: Client-server architecture with web access
Security: Role-based access control with electronic signatures
Automation: Scheduler for automated sequences and reports
Languages: English, French, German, Japanese, Chinese

Controls and Interface

Main interface components: Control Panel, Sequence Editor, Data Processing, and Report Designer.

Main Toolbar: File operations, instrument control, sequence management, data processing, reporting.

Control Panel: Real-time instrument monitoring and manual control. Sequence Editor: Create and edit acquisition sequences with method scheduling. Data Processing: Integration, calibration, quantification with manual and automated processing. Report Designer: Customizable templates with drag-and-drop components. Administration Console: User management, security settings, system configuration.

Instrument Configuration

Configure instruments through Instrument Manager for proper communication and control.

Open Instrument Manager from Administration Console
Add new instrument: Select instrument type and communication protocol
Configure communication: Set IP address, COM port, or network settings
Test connection: Verify instrument communication and control
Method mapping: Assign default methods and processing settings

Tip: Ensure instruments are powered on and connected before configuration.

Method Development

Create and optimize chromatographic methods using automated tools.

Method Wizard: Step-by-step method creation for beginners
Method Scouting: Automated screening of columns and mobile phases
Optimization: DOE-based optimization of critical parameters
Transfer: Method transfer between different instrument platforms
Validation: System suitability testing and method validation protocols
Storage: Version-controlled method storage in database
Templates: Create method templates for routine analyses
Backup: Regular method backup to prevent data loss

WARNING! Validate methods before routine use. Document all changes in audit trail.

Data Processing

Process acquired data using advanced integration and quantification algorithms.

Peak Integration: Automatic and manual integration with baseline correction. Calibration: Single-level and multi-level calibration curves. Quantification: Absolute and relative quantification with internal standards. Review: Interactive review with integration and quantification override. Reporting: Automated report generation with customizable templates.

Compliance Features

Built-in compliance tools for regulated environments including pharmaceuticals and biotechnology.

Electronic Signatures: Configurable signing workflows with reason codes. Audit Trail: Comprehensive audit trail for all data and system changes. User Management: Role-based access control with password policies. Data Integrity: Checks for data modification and deletion prevention. Backup & Archive: Automated data backup and long-term archiving.

Regulatory Information

Chromeleon 7 complies with 21 CFR Part 11 requirements for electronic records and signatures. The software incorporates security features including user authentication, audit trails, and electronic signatures. Copyright © 2024 Thermo Fisher Scientific. All trademarks are property of Thermo Fisher Scientific and its subsidiaries. For technical support, visit www.thermofisher.com or contact 1-800-532-4752.

Troubleshooting

Symptom	Possible Cause	Corrective Action
Instrument not connecting	Communication settings	Check IP/COM settings; verify instrument power and cables; restart services.
Slow performance	System resources/database	Check RAM and CPU usage; optimize database; clear temporary files.
License errors	License server/activation	Verify license server connection; reactivate license; check expiration date.
Data not saving	Database permissions	Check database connection; verify user permissions; restart database service.
Printing issues	Printer drivers/templates	Update printer drivers; check report templates; verify print permissions.

Reset: Administration Console > System > Reset Services if available.

Service: 1-800-532-4752; www.thermofisher.com