Dionex Chromeleon Software Manual

Dionex Chromeleon is a comprehensive chromatography data system software for instrument control, data acquisition, processing, and reporting. The software supports various chromatography techniques including HPLC, UHPLC, IC, and GC systems. Below are key sections covering software overview, installation, configuration, operation, data analysis, and troubleshooting.

1. Software Overview 2. System Requirements 3. Installation Guide 4. Instrument Configuration 5. Method Development 6. Data Acquisition 7. Data Processing & Analysis 8. Reporting Features 9. User Management 10. Maintenance & Updates 11. Troubleshooting

Software Overview

Chromeleon CDS provides complete workflow solutions for chromatography laboratories with intuitive interface and powerful data management capabilities.

Feature	Description
Instrument Control	Supports multiple chromatography instruments simultaneously
Data Acquisition	Real-time data collection and monitoring
Method Development	Visual method editor with drag-and-drop functionality
Data Processing	Advanced peak integration and quantification
Reporting	Customizable report templates and automated reporting
Compliance	21 CFR Part 11 compliant with audit trail
Multi-user	Role-based access control and user management
Database	Centralized data storage and management
Workflow Automation	Sequence-based automated analysis
Data Security	Encrypted data storage and backup

System Requirements

Minimum hardware and software requirements for Chromeleon installation.

Operating System: Windows 10/11 (64-bit)
Processor: Intel Core i5 or equivalent
RAM: 8 GB minimum (16 GB recommended)
Storage: 50 GB free disk space
Display: 1920x1080 resolution minimum
Network: Gigabit Ethernet for multi-user setup
Database: SQL Server 2019 or later
Browser: Internet Explorer 11 or Edge for web features

Installation Guide

Step-by-step installation procedure for Chromeleon software.

Run setup.exe as administrator from installation media
Accept license agreement and choose installation type
Select installation directory and components
Configure database connection settings
Set up system administrator account
Complete installation and restart system
Run post-installation configuration wizard

IMPORTANT! Ensure all prerequisites are met before installation. Close all other applications during installation.

Instrument Configuration

Configure and connect chromatography instruments to Chromeleon system.

Open Instrument Configuration workspace
Add new instrument using device discovery
Configure communication parameters (COM port, IP address)
Set up instrument properties and capabilities
Test instrument connection and communication
Save instrument configuration

Note: Ensure instruments are powered on and connected before configuration.

Method Development

Create and optimize chromatography methods using Chromeleon method editor.

Open Method Editor from main menu
Select instrument and create new method
Define chromatographic parameters (flow rate, gradient, temperature)
Configure detector settings (wavelength, sampling rate)
Set up data acquisition parameters
Validate method performance
Save method to method library

Data Acquisition

Run sequences and acquire chromatographic data.

Create new sequence with sample information
Assign methods and processing methods to sequence
Set sequence parameters (injection volume, run time)
Start sequence and monitor real-time data
Review acquired chromatograms
Export data for further analysis

Tip: Use sequence templates for routine analysis to save time.

Data Processing & Analysis

Process chromatographic data and perform quantitative analysis.

Processing Step	Description
Peak Integration	Automatic and manual peak detection and integration
Baseline Correction	Adjust baseline for accurate peak quantification
Calibration	Create calibration curves using standards
Quantification	Calculate sample concentrations based on calibration
System Suitability	Evaluate system performance parameters
Data Review	Review and approve processed data

Reporting Features

Generate comprehensive reports and export data.

Select report template from library
Choose data to include in report
Customize report layout and formatting
Add headers, footers, and company logo
Preview report before generation
Export to PDF, Excel, or print directly

User Management

Manage user accounts and access permissions.

User Roles: Administrator, Supervisor, Operator, Viewer
Permissions: Role-based access to features and data
Audit Trail: Complete tracking of user activities
Electronic Signatures: 21 CFR Part 11 compliant
Password Policy: Enforce strong password requirements

Maintenance & Updates

Regular maintenance tasks and software updates.

Perform regular database backups
Update software with latest patches
Archive old data to free storage space
Verify system performance regularly
Update instrument drivers as needed

CAUTION! Always backup data before performing software updates.

Troubleshooting

Issue	Possible Cause	Solution
Instrument not detected	Connection issues/drivers	Check cables; reinstall drivers; verify communication settings
Software crashes	Memory issues/corruption	Restart software; check system resources; reinstall if persistent
Data acquisition failure	Method errors/instrument	Verify method parameters; check instrument status; restart acquisition
Database connection lost	Network/SQL issues	Check network connectivity; restart SQL service; verify credentials
Performance slow	Resource constraints	Close other applications; increase RAM; optimize database

Support: Contact Thermo Fisher Scientific support at 1-800-532-4752 or visit www.thermofisher.com/chromeleon