Dionex IC 2100 Manual

The Dionex IC 2100 is a compact, high-performance ion chromatography system designed for routine analysis of anions and cations. It features a dual-piston pump with pulse dampener, conductivity detector, temperature-controlled column compartment, and Chromeleon chromatography data system compatibility. The system supports isocratic and gradient elution, automated sample injection, and various detection methods. Below are key sections for safety, features, installation, operation, maintenance, and troubleshooting.

Features at a Glance

Key components: Dual-piston pump, conductivity detector, column compartment, autosampler, Chromeleon software interface.

Installation

Unpack and inspect contents. Place on stable, level surface in laboratory environment.

1. System placement: Ensure adequate clearance for ventilation and service access.
2. Connect eluent reservoirs: Use appropriate tubing to pump inlet.
3. Connect waste line: Route to appropriate waste container.
4. Install columns: Follow manufacturer guidelines for column installation.
5. Connect to power: Use provided power cable to appropriate outlet.
6. Connect to computer: Use USB or Ethernet cable for Chromeleon interface.

WARNING! System contains high pressure components; ensure all connections are secure before operation.

First-Time Setup

Power on system; initialize Chromeleon software.

1. System priming: Prime pump with eluent to remove air bubbles.
2. Column conditioning: Condition new columns according to manufacturer specifications.
3. Detector calibration: Perform initial detector calibration.
4. Method development: Create or load analysis methods in Chromeleon.
5. System verification: Run system suitability test with standard solutions.

CAUTION! Wear appropriate personal protective equipment when handling chemicals.

Specifications

• Pump Type: Dual-piston, serial flow design
• Flow Rate Range: 0.001 to 5.0 mL/min
• Pressure Limit: 5000 psi (345 bar)
• Detector Type: Temperature-controlled conductivity
• Temperature Range: 5°C to 80°C (column compartment)
• Autosampler Capacity: 120 vials (2 mL)
• Data System: Chromeleon chromatography software
• Power Requirements: 100-240 VAC, 50/60 Hz
• Dimensions: 55 cm W × 45 cm D × 45 cm H
• Weight: 25 kg

Controls and Settings

Access system controls via Chromeleon software or front panel interface.

Front Panel: POWER, EMERGENCY STOP, STATUS INDICATORS, FUNCTION KEYS.

Pump Settings: Flow rate, pressure limits, composition (isocratic/gradient). Detector Settings: Temperature, range, auto-zero, output signal. Column Oven: Temperature setpoint, stability criteria. Autosampler: Injection volume, wash cycles, sequence programming. System: Communication settings, user access levels, method storage.

Connecting Components

Eluent lines: Pump to reservoirs. Column: Pump to detector. Waste: Detector to container. Data: System to computer.

1. Turn off system before making connections.
2. Use appropriate tubing and fittings for each connection.
3. Ensure all high-pressure connections are finger-tight plus 1/4 turn.
4. Verify no leaks at operating pressure.
5. Configure communication settings in Chromeleon.

Tip: Label all connections for easy identification during maintenance.

Operating Procedures

Supports routine ion analysis for anions and cations.

1. Method selection: Load appropriate analysis method in Chromeleon.
2. Sample preparation: Prepare samples according to method requirements.
3. Autosampler loading: Place samples in designated vials in tray.
4. Sequence setup: Define sample sequence with appropriate standards and blanks.
5. System start: Initiate sequence from Chromeleon interface.
6. Monitoring: Observe system pressure, baseline, and peak shapes.
7. Data analysis: Review chromatograms and calculate results.
8. System shutdown: Flush system with storage solution if required.

WARNING! Always use system within specified pressure limits. Monitor for leaks during operation.

Maintenance & Cleaning

Regular maintenance ensures optimal performance and extends system life.

1. Daily: Check pressures, inspect for leaks, verify baseline stability
2. Weekly: Replace inlet filters, clean autosampler needle
3. Monthly: Perform detector calibration, replace seals as needed
4. As needed: Clean injection valve, replace columns

CAUTION! Power off system before performing maintenance. Use appropriate cleaning solutions.

Regulatory Information

CE marked: Complies with European Union safety standards. FCC Part 15: Class A digital device. RoHS compliant: Meets Restriction of Hazardous Substances directive. For technical support, contact Thermo Fisher Scientific at 1-800-532-4752 or visit www.thermofisher.com.

Troubleshooting

Reset: Power cycle system; reinitialize Chromeleon if communication lost.

Service: 1-800-532-4752; www.thermofisher.com