Dionex ICS-1600 Manual

The Dionex ICS-1600 is a high-performance ion chromatography system designed for analytical laboratories, featuring advanced conductivity detection, automated eluent generation, and Chromeleon chromatography data system compatibility. Below are key sections for safety, features, installation, setup, operation, and troubleshooting.

1. Features at a Glance 2. Installation 3. First-Time Setup 4. Specifications 5. Controls and Settings 6. Connecting Devices 7. Operating Procedures 8. Maintenance Features 9. Care & Cleaning 10. Regulatory Information 11. Troubleshooting

Features at a Glance

Key components: High-pressure pump, conductivity detector, automated eluent generator, injection valve, column oven, and Chromeleon software interface.

Feature	Description
High-pressure pump	Capable of flow rates from 0.001 to 5.0 mL/min
Conductivity detector	Temperature-controlled with electronic suppression
Automated eluent generator	Generates high-purity eluents on-demand
Injection valve	Supports manual and automated injections
Column oven	Temperature range 5°C to 80°C
Chromeleon software	Complete system control and data analysis
Power input	100-240V AC, 50/60Hz
Communication	Ethernet and USB connectivity
Dimensions	55 cm W × 45 cm D × 45 cm H
Weight	Approximately 25 kg

Installation

Unpack and inspect contents. Place on stable laboratory bench.

Position system: Ensure adequate clearance for ventilation and access.
Connect solvent lines: Install eluent reservoirs and connect tubing.
Connect waste: Route waste tubing to appropriate container.
Power connection: Plug into grounded electrical outlet.
Computer connection: Connect via Ethernet or USB to control computer.

WARNING! System contains high-pressure components; ensure all connections are secure before operation.

First-Time Setup

Power on system; initialize Chromeleon software.

System priming: Purge pump and lines to remove air bubbles.
Column installation: Install and condition analytical column.
Detector calibration: Perform baseline calibration and noise test.
Method development: Create or load analytical methods.
System verification: Run quality control standards.

CAUTION! Use only HPLC-grade solvents and ultrapure water.

Specifications

Flow Rate Range: 0.001 to 5.0 mL/min
Pressure Limit: 5000 psi (345 bar)
Detector Range: 0-15,000 μS/cm
Temperature Control: 5°C to 80°C ± 0.1°C
Injection Volume: 1-100 μL
Noise Level: < 0.5 nS/cm
Data Acquisition: 100 Hz
Power Requirements: 100-240V AC, 50/60Hz, 300W
Operating Environment: 15-35°C, 20-80% relative humidity

Controls and Settings

Access system controls via Chromeleon software interface. Front Panel: POWER, STATUS indicators, emergency stop.

Software Controls: Method editor, sequence table, real-time monitoring, data processing, report generation.

Pump: Flow rate, pressure limits, composition. Detector: Range, temperature, output filter, auto-zero. Oven: Temperature, equilibration time. Injector: Injection volume, wash cycles. Eluent Generator: Concentration, refill cycles.

Connecting Devices

Solvent delivery: Eluent reservoirs, degasser. Detection: Conductivity cell, suppressor. Data: Computer interface.

Turn off system before connecting components.
Connect solvent lines using appropriate fittings.
Install column with correct orientation.
Connect detector cell and suppressor.
Establish computer communication.

Tip: Use only Dionex-approved tubing and fittings for optimal performance.

Operating Procedures

Supports isocratic and gradient elution methods.

System preparation: Prime pump, equilibrate column.
Sample injection: Load sample via manual injector or autosampler.
Method execution: Start chromatographic run.
Data acquisition: Monitor real-time chromatogram.
Peak integration: Process data using Chromeleon.
System shutdown: Flush with appropriate storage solvent.
Calibration: Run calibration standards for quantification.
Quality control: Monitor system suitability parameters.

WARNING! Always depressurize system before opening fluidic connections.

Maintenance Features

Automated diagnostics: System self-check on startup. Maintenance reminders: Scheduled component replacement alerts. Data backup: Automated method and data archiving.

Care & Cleaning

Power off before cleaning. Wipe exterior with mild detergent; clean fluidic paths with appropriate solvents.

CAUTION! Use only compatible solvents; avoid abrasive cleaners.

Regulatory Information

CE marked: Complies with European Union safety standards. FDA: Meets requirements for pharmaceutical applications. Copyright © 2025 Thermo Fisher Scientific; trademarks of Thermo Fisher Scientific. For technical support, visit www.thermofisher.com or contact 1-800-532-4752.

Troubleshooting

Symptom	Possible Cause	Corrective Action
High pressure	Column blockage	Check and replace column; flush system.
Noisy baseline	Air bubbles/contamination	Degas solvents; purge system; clean cell.
Peak tailing	Column degradation	Replace column; check mobile phase pH.
No flow	Pump seal failure	Replace pump seals; check for leaks.
Communication error	Cable/software issue	Check connections; restart software.

Reset: Software > System > Reset configuration.

Service: 1-800-532-4752; www.thermofisher.com